Real time polymerase chain reaction is a very popular technique used to measure gene expression level relative to various constitutively expressed genes such as Glyceraldehyde-3 phosphate dehydrogenase (GAPDH), beta-actin. It is based on the binding of SYBR green dye to double stranded DNA (dsDNA) as the amplification of complementary DNA (cDNA) takes place in PCR. SYBR green dye binds to dsDNA and fluoresce in the visible range emitting green color, thus the name SYBR green.
It consists of various preparative steps
1 mRNA extraction
2 cDNA preparation
3 RT-PCR
It consists of various preparative steps
1 mRNA extraction
2 cDNA preparation
3 RT-PCR
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