Proliferation assays as the name suggests are used to the estimate the proliferation of cells in the sample. Measuring cellular proliferation is important as in many pathological (diseased) and physiological (normal) conditions, proliferation varies depending on the situation. For instance, vascular smooth muscle cells, a type of cells found in the middle layer of blood vessels (arteries) proliferate as a part of disease of blood vessels (atherosclerosis). Proliferation assays measures metabolic rate of the cell more or less which is infact an index of the growing or dying or fixed situation of the cell. Metabolic rate is measured through activity of an enzyme converted in the form of a visible dye which is quantified by taking absorbance at a certain wavelength. These assays are very sensitive as they can detect very less change in cell. Some of the popular versions of proliferation assays are listed below.
MTT Assay
Alamar blue Assay
No comments:
Post a Comment